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Proceedings Paper

Planar plasmonic antenna arrays resolve transient nanoscopic heterogeneities in biological membranes
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Paper Abstract

We introduce an innovative design of planar plasmonic nanogap antenna arrays and demonstrate its potential to study the spatiotemporal organization of mimetic biological membranes at the nanoscale. We exploit our novel nanogap antenna platform with different nanogap sizes (10-45 nm) combined with fluorescence correlation spectroscopy to reveal the existence of nanoscopic domains in mimetic biological membranes. Our approach takes advantage of the highly enhanced and confined excitation light provided by the antennas together with their extreme planarity to investigate membrane regions as small as 10 nm in size with microsecond temporal resolution. We first demonstrate the ultra-high confinement of photonic antennas on biological membranes. Moreover, we show that cholesterol slows down the diffusion of individual fluorescent molecules embedded in the lipid bilayer, consistent with the formation of nanoscopic domains enriched by cholesterol. Incorporation of hyaluronic acid (HA) to the ternary lipid mixture further slows down molecular diffusion, suggesting a synergistic effect of cholesterol and HA on the dynamic partitioning of mimetic biological membranes.

Paper Details

Date Published: 13 February 2020
PDF: 8 pages
Proc. SPIE 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII, 112460F (13 February 2020); doi: 10.1117/12.2543726
Show Author Affiliations
Pamina M. Winkler, ICFO - Institut de Ciències Fotòniques (Spain)
Felix Campelo, ICFO - Institut de Ciències Fotòniques (Spain)
Marina I. Giannotti, Ctr. de Investigación Biomédica en Red (CIBER), Institut de Bioenginyeria de Catalunya (IBEC) (Spain)
María F. García-Parajo, ICFO - Institut de Ciències Fotòniques (Spain)
ICREA (Spain)

Published in SPIE Proceedings Vol. 11246:
Single Molecule Spectroscopy and Superresolution Imaging XIII
Ingo Gregor; Felix Koberling; Rainer Erdmann, Editor(s)

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