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Proceedings Paper

STED imaging of cytoplasmic RNA in live cells
Author(s): Jianguo Zhang; Ying He; Zhenyu Gu; Dan Zhang; Pintu Das; Junle Qu; Zhigang Yang
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Paper Abstract

RNAs play pivotal roles in many biological processes, such as translation, gene editing etc. Since RNAs are usually smaller in size than optical diffraction limit, it is impossible for optical microscopes to get deep insight of them. With the recent emergence of super-resolution optical imaging microscopies, it becomes possible to study the ultra-fine structure of such RNA in side cells. Different kind of fluorescent probes are used for RNA labeling and imaging inside cells. However, most of them cannot be used for live-cell super-resolution imaging, due to typical laser toxicity to live cells. Herein, we developed a new type of RNA-labeled probe, which can be used for STED imaging of RNA in living cells. We believe this probe is of great significance for studying biological behaviors of RNA in living cells.

Paper Details

Date Published: 21 February 2020
PDF: 8 pages
Proc. SPIE 11254, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII, 1125411 (21 February 2020); doi: 10.1117/12.2543706
Show Author Affiliations
Jianguo Zhang, Shenzhen Univ. (China)
Ying He, Shenzhen Univ. (China)
Zhenyu Gu, Shenzhen Univ. (China)
Dan Zhang, Shenzhen Univ. (China)
Pintu Das, Shenzhen Univ. (China)
Junle Qu, Shenzhen Univ. (China)
Zhigang Yang, Shenzhen Univ. (China)


Published in SPIE Proceedings Vol. 11254:
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII
Dror Fixler; Ewa M. Goldys, Editor(s)

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