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Proceedings Paper

Full-field interferometric imaging of neuron deformation during action potential (Conference Presentation)

Paper Abstract

We report the full-field imaging of the mechanical deformations accompanying the action potential in primary cortical neurons using ultrafast quantitative phase imaging (QPI) with a temporal resolution of 0.1 ms and a membrane displacement sensitivity of <0.2 nm per pixel. The average displacements were ~0.7 nm on cell somas and ~0.5 nm on neurites. Finite element modeling based on the 3D shape extracted from confocal imaging and on scaling of the surface tension with trans-membrane voltage yielded the deformation map during action potential, which matched the features of the experimental results, including the displacement amplitude, time course, and spatial distribution.

Paper Details

Date Published: 10 March 2020
Proc. SPIE 11251, Label-free Biomedical Imaging and Sensing (LBIS) 2020, 112511Q (10 March 2020); doi: 10.1117/12.2543625
Show Author Affiliations
Tong Ling, Stanford Univ. (United States)
Kevin C. Boyle, Stanford Univ. (United States)
Valentina Zuckerman, Stanford Univ. (United States)
Thomas Flores, Stanford Univ. (United States)
Daniel V. Palanker, Stanford Univ. (United States)

Published in SPIE Proceedings Vol. 11251:
Label-free Biomedical Imaging and Sensing (LBIS) 2020
Natan T. Shaked; Oliver Hayden, Editor(s)

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