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Proceedings Paper

High-speed, high-resolution mesoscopic multiphoton microscopy of human skin (Conference Presentation)
Author(s): Alexander Fast; Anand Ganesan; Kristen Kelly; Christopher Zachary; Mihaela Balu

Paper Abstract

Multiphoton microscopy (MPM) can provide sub-micron resolution images of living tissues in their native environment with contrast from multiple modalities, including second harmonic generation (SHG) and two-photon excited fluorescence (TPEF). Recent advances of MPM in clinical skin imaging demonstrated the unique potential of this technology as a label-free research and clinical tool for a broad area of applications such as melanoma and non-melanoma skin cancer detection, monitoring pigmentary skin disorders, characterizing keratinocyte metabolism, etc. In this contribution we demonstrate the ability of this microscope to provide sub-micrometer resolution ex-vivo images of large areas of skin tissue (up to 5x5 mm2) in <1 minute. We demonstrate the importance of high-speed, high-resolution mesoscopic imaging on cancerous skin tissues that present heterogeneous morphology to show the ability of the instrument to capture both benign and malignant areas of the lesion.

Paper Details

Date Published: 6 March 2020
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Proc. SPIE 11211, Photonics in Dermatology and Plastic Surgery 2020, 112110F (6 March 2020); doi: 10.1117/12.2543603
Show Author Affiliations
Alexander Fast, Beckman Laser Institute and Medical Clinic (United States)
Anand Ganesan, Univ. of California, Irvine (United States)
Kristen Kelly, Univ. of California, Irvine (United States)
Christopher Zachary, Univ. of California, Irvine (United States)
Mihaela Balu, Beckman Laser Institute and Medical Clinic (United States)


Published in SPIE Proceedings Vol. 11211:
Photonics in Dermatology and Plastic Surgery 2020
Bernard Choi; Haishan Zeng, Editor(s)

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