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Proceedings Paper

Computational microscopy for quantitative phase imaging and refractive index tomography using annular illumination
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Paper Abstract

In this work, we demonstrate a computational microscopy for quantitative phase imaging and refractive index tomography using annular illumination. By employing a physical annular plate or a programmable annular LED unit, the quantitative phase images of a thin phase object or large-volume three-dimensional (3D) refractive index (RI) distributions of thick object can be rapidly characterize and recovered. This annular illumination scheme optimally encodes both low- and high-spatial frequency for quantitative phase and RI information across the entire 3D volume using a small number of intensity measurements. We also give both quantitative phase and 3D RI experiment results based on various biological samples, and this computational microscopy approach shows promise as a powerful high-speed, label-free tool for biomedical applications and possibility of widespread adoption of phase imaging in the morphology study of cellular processes and biomedical community.

Paper Details

Date Published: 14 February 2020
PDF: 8 pages
Proc. SPIE 11249, Quantitative Phase Imaging VI, 112491F (14 February 2020); doi: 10.1117/12.2543597
Show Author Affiliations
Jiaji Li, Nanjing Univ. of Science and Technology (China)
Alex Matlock, Boston Univ. (United States)
Yunzhe Li, Boston Univ. (United States)
Qian Chen, Nanjing Univ. of Science and Technology (China)
Lei Tian, Boston Univ. (United States)
Chao Zuo, Nanjing Univ. of Science and Technology (China)


Published in SPIE Proceedings Vol. 11249:
Quantitative Phase Imaging VI
Yang Liu; Gabriel Popescu; YongKeun Park, Editor(s)

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