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Proceedings Paper

Self-interference digital holography (SIDH) for far-field three-dimensional nanoscale localization of fluorescent nanoparticles
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Paper Abstract

Self-interference digital holography (SIDH) is a promising approach for three-dimensional imaging as it offers the ability to view a complete three-dimensional volume from a single image. SIDH has so far, largely been limited to image samples that emit a large number of photons. We report the use of a Michelson interferometer based SIDH setup which provides higher light throughput compared to previous systems that employed spatial light modulators (SLM). SIDH microscopy incorporating a Michelson interferometer in the proposed optical configuration and high-numerical-aperture oil immersion objectives can be used to perform super-resolution single-molecule localization microscopy (SMLM) and single-particle tracking (SPT) over large axial ranges. We demonstrate this by localizing a single 0.1 µm diameter fluorescent nanosphere using a custom built wide-field microscope. With 49,000 photons detected, the proposed system achieves a localization precision of 4.5 nm in x, 5 nm in y and 39.8 nm in z over a 20 µm axial range. Further, we also discuss the SNR requirements to image photon-limited light sources such as a single-molecules using SIDH.

Paper Details

Date Published: 13 February 2020
PDF: 10 pages
Proc. SPIE 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII, 112460M (13 February 2020); doi: 10.1117/12.2543422
Show Author Affiliations
Abhijit Marar, Univ. of Georgia (United States)
Peter Kner, Univ. of Georgia (United States)

Published in SPIE Proceedings Vol. 11246:
Single Molecule Spectroscopy and Superresolution Imaging XIII
Ingo Gregor; Felix Koberling; Rainer Erdmann, Editor(s)

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