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Proceedings Paper

Biosensing the presence of nanoparticles using endogenous fluorescence in live algae
Author(s): A. Marcek Chorvatova; T. Teplicky; M. Valica; A. Mateasik; D. Chorvat Jr.
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Paper Abstract

Nanoparticles (NPs) from various metals (Zinc, Nickel, Cobalt, Copper) were designed and fabricated by direct synthesis using femtosecond laser ablation in liquids. Employing confocal microscopy with spectral detection and fluorescence lifetime imaging (FLIM), we have evaluated interaction of fabricated NPs with living Chlorella sp. algae by means of their naturally presented endogenous fluorescence. Live cell imaging was done in spectral region 500-550 nm and 650- 710 nm to evaluate the effect of NPs on both, the green and the red fluorescence that is derived from flavonoids/carotenoids and chlorophylls respectively. We observed fluorescence intensity decrease in the red spectral region by all but Ni NPs. The presence of NPs also lead to an increase in the blue fluorescence at 477-488 nm, possibly resulting from reflected light. Gathered observations constitute the first step towards creation of methodological approaches for fast natural biosensing of the effects of environmental pollution directly in live algae.

Paper Details

Date Published: 21 February 2020
PDF: 10 pages
Proc. SPIE 11254, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII, 1125402 (21 February 2020); doi: 10.1117/12.2543332
Show Author Affiliations
A. Marcek Chorvatova, International Laser Ctr. (Slovakia)
Ss. Cyril and Methodius Univ. (Slovakia)
T. Teplicky, Comenius Univ. (Slovakia)
M. Valica, Ss. Cyril and Methodius Univ. (Slovakia)
A. Mateasik, International Laser Ctr. (Slovakia)
D. Chorvat Jr., International Laser Ctr. (Slovakia)

Published in SPIE Proceedings Vol. 11254:
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII
Dror Fixler; Ewa M. Goldys, Editor(s)

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