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Proceedings Paper

Epi-illumination SPIM (eSPIM) for high-throughput volumetric imaging (Conference Presentation)
Author(s): Bo Huang

Paper Abstract

Selective-plane illumination microscopy (SPIM) are particularly advantageous in long-term live volumetric imaging, although its common geometry is incompatible with common biological sample holders, including multi-well plates. To solve this problem, we designed an epi-illumination SPIM (eSPIM) system with high spatial resolution and light collection efficiency with single-molecule sensitivity. It has an identical sample interface as an inverted fluorescence microscope with a single primary objective and no additional reflection elements. We have demonstrated multicolor, fast, volumetric imaging of live cells as well as single-molecule super-resolution microscopy on eSPIM. Moreover, it has enabled long term imaging of cells in parallel in multiwell plates and simultaneous recording of cellular responses to a panel of perturbations.

Paper Details

Date Published: 10 March 2020
Proc. SPIE 11250, High-Speed Biomedical Imaging and Spectroscopy V, 1125002 (10 March 2020); doi: 10.1117/12.2543259
Show Author Affiliations
Bo Huang, Univ. of California, San Francisco (United States)
Chan Zuckerberg Biohub (United States)

Published in SPIE Proceedings Vol. 11250:
High-Speed Biomedical Imaging and Spectroscopy V
Kevin K. Tsia; Keisuke Goda, Editor(s)

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