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Proceedings Paper

Sensorless and sensor based adaptive optics for light sheet microscopy
Author(s): Yang Liu; Keelan Lawrence; James D. Lauderdale; Peter Kner
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Paper Abstract

Light Sheet Microscopy has developed rapidly over the past decade and is the ideal approach for imaging model organisms such as zebrafish and other thick tissue specimens. Despite the superior optical sectioning capability, high imaging speed, and large field of view, the performance of light sheet microscopy still suffers from optical aberrations. We have implemented a scene-based Shack-Hartmann wavefront sensor for directly measuring the optical aberrations on the emission side of the light-sheet microscope. In this work, we show that our system is capable of AO correction using sensor based and sensorless based approaches. We demonstrate correction up-to one hundred microns deep in zebrafish and fruitfly embryos.

Paper Details

Date Published: 17 February 2020
PDF: 7 pages
Proc. SPIE 11248, Adaptive Optics and Wavefront Control for Biological Systems VI, 1124806 (17 February 2020); doi: 10.1117/12.2543251
Show Author Affiliations
Yang Liu, The Univ. of Georgia (United States)
Keelan Lawrence, The Univ. of Georgia (United States)
James D. Lauderdale, The Univ. of Georgia (United States)
Peter Kner, The Univ. of Georgia (United States)


Published in SPIE Proceedings Vol. 11248:
Adaptive Optics and Wavefront Control for Biological Systems VI
Thomas G. Bifano; Sylvain Gigan; Na Ji, Editor(s)

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