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Proceedings Paper

Multiplexed 3-photon microscopy for functional connectomics of mammalian brains
Author(s): Kevin T. Takasaki; Dmitri Tsyboulski; Jack Waters
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Paper Abstract

3-photon excitation enables in vivo fluorescence microscopy deep in densely labeled and highly scattering samples, while maintaining high resolution and contrast. We designed and characterized a dual-plane 3-photon microscope with temporal multiplexing and remote focusing, and performed simultaneous in vivo calcium imaging of two planes deep in the cortex of a transgenic mouse expressing GCaMP6s in nearly all excitatory neurons.

Paper Details

Date Published: 14 February 2020
PDF: 4 pages
Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 112441F (14 February 2020); doi: 10.1117/12.2543232
Show Author Affiliations
Kevin T. Takasaki, Allen Institute for Brain Science (United States)
Dmitri Tsyboulski, Howard Hughes Medical Institute (United States)
Jack Waters, Allen Institute for Brain Science (United States)

Published in SPIE Proceedings Vol. 11244:
Multiphoton Microscopy in the Biomedical Sciences XX
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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