Label-free multiphoton imaging has been a powerful tool to study the microstructure and specific chemical distributions in biological tissue, especially in tumors and their microenvironments. Thus, this technique has great potential to assist in cancer-related clinical studies. A portable label-free multiphoton imaging system was constructed with four imaging modalities: two- and three- photon fluorescence, and second and third harmonic generation. Mosaicked multimodal images can be acquired with dual-channel detection and galvo-mirror scanning. This system was demonstrated during animal surgeries for real-time, label-free assessment of tumor tissue samples acquired via core-needle biopsy and fine-needle aspiration.

Date Published: 9 March 2020
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Proc. SPIE 11234, Optical Biopsy XVIII: Toward Real-Time Spectroscopic Imaging and Diagnosis, 112340X (9 March 2020); doi: 10.1117/12.2543216

Published in SPIE Proceedings Vol. 11234:
Optical Biopsy XVIII: Toward Real-Time Spectroscopic Imaging and Diagnosis
Robert R. Alfano; Stavros G. Demos; Angela B. Seddon, Editor(s)