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Proceedings Paper

Large area functional and structural nonlinear brain imaging (Conference Presentation)
Author(s): Francesco Saverio Pavone

Paper Abstract

In this work large area reconstruction are obtained using a mesoscale two-photon system for structural analysis and a light sheet two-photon microscope for functional information. The mesoscale methodology developed allows analyzing the cytoarchitecture of the human brain in three dimensions at high resolution. Functional imaging has been used to investigate whole organ, like zebrafish larval brain activity, using standard scanning or light sheet two-photon illumination. Both modalities are capable to sample whole brain with single cell resolution, with light sheet imaging being capable to perform high rate volumetric imaging allowing to map in real time whole-brain calcium dynamics not affected by undesired visual stimulation artefacts, as occurring in one photon excitation fluorescence microscopy. Large area non linear imaging allows in general extended measurements of the neuronal activity in normal conditions under pathological modeling of epilepsy and during visual stimulation.

Paper Details

Date Published: 9 March 2020
Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 112440W (9 March 2020); doi: 10.1117/12.2543177
Show Author Affiliations
Francesco Saverio Pavone, LENS - Lab. Europeo di Spettroscopie Non-Lineari (Italy)

Published in SPIE Proceedings Vol. 11244:
Multiphoton Microscopy in the Biomedical Sciences XX
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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