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Proceedings Paper

Multicolor spatial cumulant analysis to measure dopamine receptor dynamics on the plasma membrane (Conference Presentation)
Author(s): Daniel J. Foust; Allesandro Ustione; David Piston

Paper Abstract

Two-photon excitation facilitates fluorescence fluctuation spectroscopy experiments by providing a well-defined observation volume that is identical across multiple emitting species. We have developed a multi-color approach, spatial cumulant analysis (SpCA), which provides images of molecular stoichiometries. We have used SpCA to determine the dynamics of dopamine receptors (DR) on the insulin secreting β-cells. There are two inhibitory DRs in the β-cell, D2 and D3, which differentially impact cAMP production, Ca2+ influx, and K+ currents. We show how multicolor spatial cumulant analysis of labeled proteins of interest can accurately measure the stoichiometry of receptors, G-proteins, and downstream signaling targets.

Paper Details

Date Published: 9 March 2020
Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 1124406 (9 March 2020); doi: 10.1117/12.2543117
Show Author Affiliations
Daniel J. Foust, Washington Univ. in St. Louis (United States)
Allesandro Ustione, Washington Univ. in St. Louis (United States)
David Piston, Washington Univ. in St. Louis (United States)

Published in SPIE Proceedings Vol. 11244:
Multiphoton Microscopy in the Biomedical Sciences XX
Ammasi Periasamy; Peter T. C. So; Karsten König, Editor(s)

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