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Proceedings Paper

Fiber-based macroscale fluorescence lifetime imaging for real-time in situ tissue diagnostics (Conference Presentation)
Author(s): Joao Lagarto; Vladislav Shcheslavskiy; Francesco S. Pavone; Riccardo Cicchi

Paper Abstract

The inability to efficiently separate autofluorescence signals from bright background illumination is a key issue that hinders widespread deployment of optical techniques such as time-correlated single photon counting (TCSPC) in clinical settings. We introduce a novel approach to TCSPC measurements that synchronizes the fluorescence acquisition with an externally modulated light source at 50 Hz, thus guaranteeing background-free fluorescence detection and “continuous” eye-perceived specimen illumination. A color camera synchronized with fluorescence detection provides spatial resolution to the single-point measurements, to generate autofluorescence lifetime maps in real-time. We believe this method provides a new research pathway for clinical translation of optical techniques.

Paper Details

Date Published: 9 March 2020
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Proc. SPIE 11234, Optical Biopsy XVIII: Toward Real-Time Spectroscopic Imaging and Diagnosis, 112340Q (9 March 2020); doi: 10.1117/12.2543010
Show Author Affiliations
Joao Lagarto, Istituto Nazionale di Ottica, Consiglio Nazionale delle Ricerche (Italy)
Vladislav Shcheslavskiy, Becker & Hickl GmbH (Germany)
Francesco S. Pavone, Istituto Nazionale di Ottica, Consiglio Nazionale delle Ricerche (Italy)
Riccardo Cicchi, Istituto Nazionale di Ottica, Consiglio Nazionale delle Ricerche (Italy)


Published in SPIE Proceedings Vol. 11234:
Optical Biopsy XVIII: Toward Real-Time Spectroscopic Imaging and Diagnosis
Robert R. Alfano; Stavros G. Demos; Angela B. Seddon, Editor(s)

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