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Proceedings Paper

Pilot study of micro optical coherence tomography for detection of invasive and pre-invasive cervical lesions (Conference Presentation)
Author(s): Elham Abouei; Joseph A. Gardecki; Michele Follen; Calum MacAulay; Jed Cutler; Lisa Ricketts-Holcomb; Guillermo J. Tearney

Paper Abstract

Antibiotic resistance is a burgeoning global public health threats of our time. Antibiotic resistance is a multifactorial and complex problem which cannot be solved by only developing stronger and better antibiotic compounds. Rapid detection and characterization of pathogenic bacteria are critical for effectively treating bacterial infections without exacerbating the resistance problem. Here, we present a novel highly-sensitive and label-free platform, Rapid-Ultra-Sensitive-Detector (RUSD), that utilizes the high reflectance coefficient of light at the interface between low-refractive-index and high-refractive-index media. The sensitivity of RUSD is three to four orders of magnitude higher than conventional optical density-based methods. Utilizing RUSD, we can detect as low as ~20 bacterial cells or a single fungal cell. This technique does not require any sophisticated signal processing steps and it enables growth rate measurements in less than an hour. Finally, we can now measure antibiotics resistance of several gram-negative and gram-positive bacteria, including Staphylococcus aureus, Pseudomonas aeruginosa, and Escherichia coli, within two hours.

Paper Details

Date Published: 9 March 2020
Proc. SPIE 11234, Optical Biopsy XVIII: Toward Real-Time Spectroscopic Imaging and Diagnosis, 1123412 (9 March 2020); doi: 10.1117/12.2542961
Show Author Affiliations
Elham Abouei, The Univ. of British Columbia (Canada)
Joseph A. Gardecki, Massachusetts General Hospital (United States)
Michele Follen, King's County Hospital (United States)
Calum MacAulay, The Univ. of British Columbia (United States)
Jed Cutler, King's County Hospital (United States)
Lisa Ricketts-Holcomb, King's County Hospital (United States)
Guillermo J. Tearney, Harvard Medical School (United States)

Published in SPIE Proceedings Vol. 11234:
Optical Biopsy XVIII: Toward Real-Time Spectroscopic Imaging and Diagnosis
Robert R. Alfano; Stavros G. Demos; Angela B. Seddon, Editor(s)

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