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Proceedings Paper

Sensorless adaptive optics with a laser free spinning disk confocal microscope
Author(s): S. A. Hussain; T. Kubo; N. Hall; K. Hampson; M. A. Phillips; D. Gala; I. M. Dobbie; J. Antonello; M. Wincott; I. Davis; M. J. Booth
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Paper Abstract

Specimen induced aberrations can have detrimental effects in all types of high-resolution microscope. In this study, we present a sensorless technique that uses a deformable mirror (DM) to correct aberrations of both the system and sample. Using a laser-free confocal microscope, with patterned disk illumination and detection. The system is based on a commercial confocal module (Clarity, Aurox Ltd., UK) that uses Light Emitting Diode (LED) illumination to obtain optically sectioned 3D images. The results obtained show that the setup was able to correct aberrations of biological samples used in the study. These systems will help researchers working on various biological systems to obtain improved quality images when focussing deep into thick specimens.

Paper Details

Date Published: 17 February 2020
PDF: 5 pages
Proc. SPIE 11248, Adaptive Optics and Wavefront Control for Biological Systems VI, 112480W (17 February 2020); doi: 10.1117/12.2542959
Show Author Affiliations
S. A. Hussain, Univ. of Oxford (United Kingdom)
T. Kubo, Osaka Univ. (Japan)
N. Hall, Univ. of Oxford (United Kingdom)
K. Hampson, Univ. of Oxford (United Kingdom)
M. A. Phillips, Univ. of Oxford (United Kingdom)
D. Gala, Univ. of Oxford (United Kingdom)
I. M. Dobbie, Univ. of Oxford (United Kingdom)
J. Antonello, Univ. of Oxford (United Kingdom)
M. Wincott, Univ. of Oxford (United Kingdom)
I. Davis, Univ. of Oxford (United Kingdom)
M. J. Booth, Univ. of Oxford (United Kingdom)

Published in SPIE Proceedings Vol. 11248:
Adaptive Optics and Wavefront Control for Biological Systems VI
Thomas G. Bifano; Sylvain Gigan; Na Ji, Editor(s)

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