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Proceedings Paper

In vivo quantitative molecular absorption of glycerol in human skin using coherent anti-Stokes Raman scattering (CARS) and two-photon auto-fluorescence (Conference Presentation)
Author(s): Hervé Rigneault; Barbara Sarri; Xueqin Chen; Sébastien Grégoire; Jean-Baptiste Galey; Anne Potter; Thomas Bornschlögl

Paper Abstract

We introduce and validate a framework for imaging and quantifying active molecule penetration into human skin in vivo. Our approach combines nonlinear imaging microscopy modalities, such as two-photon excited auto-fluorescence (TPEF) and coherent anti-Stokes Raman scattering (CARS), together with the use of deuterated active molecules. The imaging framework is exemplified on topically applied glycerol diluted in various vehicles such as water and xanthan gel. In vivo glycerol quantitative percutaneous penetration over time is demonstrated, showing that, contrary to water, xanthan gel vehicle acts as a film reservoir that releases glycerol continuously over time.

Paper Details

Date Published: 20 April 2020
Proc. SPIE 11219, Visualizing and Quantifying Drug Distribution in Tissue IV, 1121905 (20 April 2020); doi: 10.1117/12.2542701
Show Author Affiliations
Hervé Rigneault, Institut Fresnel (France)
Barbara Sarri, Institut Fresnel (France)
Xueqin Chen, L'Oréal Recherche et Innovation (France)
Sébastien Grégoire, L'Oréal Recherche et Innovation (France)
Jean-Baptiste Galey, L'Oréal Recherche et Innovation (France)
Anne Potter, L'Oréal Recherche et Innovation (France)
Thomas Bornschlögl, L'Oréal Recherche et Innovation (France)

Published in SPIE Proceedings Vol. 11219:
Visualizing and Quantifying Drug Distribution in Tissue IV
Kin Foong Chan; Conor L. Evans, Editor(s)

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