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Proceedings Paper

Oblique plane microscope plate reader for time lapse 3D imaging of live cells in collagen (Conference Presentation)

Paper Abstract

Light sheet fluorescence microscopy (LSFM) enables fast 3D imaging of live cells, however the traditional LSFM geometry is not compatible with conventional multiwell plates used in high content microscopy. We have developed an automated LSFM platereader based on an oblique plane microscope (OPM) that is compatible with multiwell plates. The system enables automated studies of cells cultured in a collagen matrix. Cells across 30 different conditions are imaged every 5 minutes for 12 hours. A custom 3D segmentation and tracking pipeline analyses cell morphological dynamics, allowing the study of a range of treatment conditions on the ability of cancer cells to change shape and invade.

Paper Details

Date Published: 9 March 2020
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Proc. SPIE 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII, 112430Z (9 March 2020); doi: 10.1117/12.2542691
Show Author Affiliations
Nathan Curry, Imperial College London (United Kingdom)
Hugh Sparks, Imperial College London (United Kingdom)
Lucas Dent, The Institute of Cancer Research (United Kingdom)
Vicky Bousgouni, The Institute of Cancer Research (United Kingdom)
Vincent Maioli, Imperial College London (United Kingdom)
Ranjeet Kumar, Imperial College London (United Kingdom)
Sunil Kumar, Imperial College London (United Kingdom)
Chris Bakal, The Institute of Cancer Research (United Kingdom)
Christopher W. Dunsby, Imperial College London (United Kingdom)


Published in SPIE Proceedings Vol. 11243:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII
Daniel L. Farkas; Attila Tarnok, Editor(s)

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