Share Email Print
cover

Proceedings Paper

Using quantitative phase imaging to acquire angular scattering information from single macrophages (Conference Presentation)
Author(s): Robert L. Draham; Kaitlin J. Dunn; Elizabeth A. M. Lister; Jonathan J. Pinney; Michael R. Elliott; Andrew J. Berger

Paper Abstract

Angle-resolved light scattering microscopy enables the size distribution of scatterers within a cell to be estimated non-invasively. Our group is working towards obtaining quantitative estimates of mean scatterer size in single cells by comparing the angular distribution of scattered light to Mie theory models. Using quantitative phase imaging, we are able to measure multiple cells at a time and still obtain each cell’s scattering pattern individually. We are now acquiring data from macrophages performing antibody-dependent cellular phagocytosis (ADCP) in hopes of relating the size estimates to the different known stages of the process.

Paper Details

Date Published: 10 March 2020
PDF
Proc. SPIE 11253, Biomedical Applications of Light Scattering X, 112530F (10 March 2020); doi: 10.1117/12.2542684
Show Author Affiliations
Robert L. Draham, The Institute of Optics, Univ. of Rochester (United States)
Kaitlin J. Dunn, The Institute of Optics, Univ. of Rochester (United States)
Elizabeth A. M. Lister, Carnegie Mellon Univ. (United States)
The Institute of Optics, Univ. of Rochester (United States)
Jonathan J. Pinney, Univ. of Rochester (United States)
Michael R. Elliott, Univ. of Rochester (United States)
Andrew J. Berger, The Institute of Optics, Univ. of Rochester (United States)
Univ. of Rochester (United States)


Published in SPIE Proceedings Vol. 11253:
Biomedical Applications of Light Scattering X
Adam Wax; Vadim Backman, Editor(s)

© SPIE. Terms of Use
Back to Top
PREMIUM CONTENT
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?
close_icon_gray