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Proceedings Paper

Scanning FCS and super-resolution microscopy on 2D lipid membranes (Conference Presentation)

Paper Abstract

Scanning FCS (sFCS) is a great tool for studying slowly diffusing species as is often the case in cell membranes. In sFCS, the excitation volume is scanned rapidly through the sample allowing for simultaneous measurement at multiple locations. The shorter residence times also lead to lower photon doses experienced by each detected molecule, reducing the risk of photobleaching. Here, we show results from sFCS measurements on supported lipid bilayers (SLBs) where fluorescence lifetime information is used to achieve an axial nanometric localization based on Metal Induced Energy Transfer (MIET).

Paper Details

Date Published: 26 March 2020
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Proc. SPIE 11246, Single Molecule Spectroscopy and Superresolution Imaging XIII, 1124607 (26 March 2020); doi: 10.1117/12.2542417
Show Author Affiliations
Uwe Ortmann, PicoQuant GmbH (Germany)
Mariano Gonzalez Pisfil, PicoQuant GmbH (Germany)
Humboldt-Univ. (Germany)
Marcelle König, PicoQuant GmbH (Germany)
Rhys Dowler, PicoQuant GmbH (Germany)
Benedikt Krämer, PicoQuant GmbH (Germany)
Sumeet Rohilla, PicoQuant GmbH (Germany)
Felix Koberling, PicoQuant GmbH (Germany)
Rainer Erdmann, PicoQuant GmbH (Germany)


Published in SPIE Proceedings Vol. 11246:
Single Molecule Spectroscopy and Superresolution Imaging XIII
Ingo Gregor; Felix Koberling; Rainer Erdmann, Editor(s)

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