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Proceedings Paper

Laser speckle time-series correlation analysis for bacteria activity detection
Author(s): Ilya Balmages; Dmitrijs Bliznuks; Janis Liepins; Stivens Zolins; Alexey Lihachev
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Paper Abstract

The study aims at development and laboratory approbation of non-contact optical technique for early evaluation of microbial activity. Microorganisms’ activity is estimated by laser speckle contrast imaging technique in combination with image processing of obtained time varying speckle patterns. Laser speckle patterns were captured by CMOS sensor during illumination of growing bacteria colonies by low power (<30 mW, 635 nm) stabilized coherent light source. To validate proposed technique and image processing algorithm the vibrio natriegens bacteria are used. After analysis of several different experiments the following results were obtained: In the central part of the colony activity can be seen in 2.5-3 hours. Thus, earlier detection of bacterial activity is expected, i.e., earlier than 2-3 hours, which is much earlier than the standard counting methods used to count colony forming units (CFU).

Paper Details

Date Published: 1 April 2020
PDF: 9 pages
Proc. SPIE 11359, Biomedical Spectroscopy, Microscopy, and Imaging, 113591D (1 April 2020); doi: 10.1117/12.2541663
Show Author Affiliations
Ilya Balmages, Univ. of Latvia (Latvia)
Dmitrijs Bliznuks, Riga Technical Univ. (Latvia)
Janis Liepins, Univ. of Latvia (Latvia)
Stivens Zolins, Univ. of Latvia (Latvia)
Alexey Lihachev, Univ. of Latvia (Latvia)

Published in SPIE Proceedings Vol. 11359:
Biomedical Spectroscopy, Microscopy, and Imaging
Jürgen Popp; Csilla Gergely, Editor(s)

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