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Proceedings Paper

The development of a mechano-biological assessment of leukemia cells using optical tweezers
Author(s): Eric Brost; Jamison Brooks; Adam Green; Susanta Hui
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Paper Abstract

Patients with B-ALL (Ph +ve) acute lymphoblastic leukemia are at high risk of relapse and mortality. We seek to establish a mechano-biological testing technique to assess biophysical properties of cell motility, potentiating the ability to distinguish between high and low risk leukemia populations and behaviors. Two experiments were performed to test the mechano-biological behavior of B-ALL using the ThorLabs modular optical tweezers and a microfluid chemotaxis chamber. The first experiment measured the relative mechanical energy carried by a cell in the optical trap, which was performed on B-ALL in control conditions and under SDF1 chemotaxis. The relative mechanical energy was found through an extension of the back-focal-plane calibration method for optical tweezers, and assumed that increased cellular activity manifests as random movements. There was no discernable difference in the relative mechanical energies between the control B-ALL, B-ALL under SDF1 chemotaxis, and B-ALL in the presence of mesenchymal stem cells. The second experiment quantified the real-time migrational force of B-ALL under SDF1 chemotaxis. This was found through prior calibration of the optical tweezers through determination of the terms κ and β. Of the viable measurements, 3 of 8 cells exhibited a significant force towards the SDF1 gradient. Further experimentation is necessary to normalize the experimental set-up conditions, and increase the number of viable measurements during cell migration.

Paper Details

Date Published: 17 February 2020
PDF: 13 pages
Proc. SPIE 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII, 112431Z (17 February 2020); doi: 10.1117/12.2541338
Show Author Affiliations
Eric Brost, Mayo Clinic (United States)
Jamison Brooks, Univ. of Minnesota, Minneapolis (United States)
City of Hope National Medical Ctr. (United States)
Adam Green, Univ. of Saint Thomas (United States)
Susanta Hui, Univ. of Minnesota, Minneapolis (United States)
City of Hope National Medical Ctr. (United States)


Published in SPIE Proceedings Vol. 11243:
Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII
Daniel L. Farkas; Attila Tarnok, Editor(s)

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