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Proceedings Paper

High precision pH measurements in biological environments using a portable optical fibre pH sensor
Author(s): Georgina M. Sylvia; Erik P. Schartner; Hanna J. McLennan; Avishkar Saini; Kylie R. Dunning; Malcolm S. Purdey; Andrew D. Abell; Jeremy G. Thompson
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Paper Abstract

We have demonstrated that an optical fibre-based pH sensor can be utilised to accurately assess pH in a biological environment. Initial measurements were performed on 5 μL drops of culture medium containing individual female mouse reproductive cells (cumulus-oocyte-complexes, COCs), with the goal of obtaining a biomarker of individual cell health during assisted reproductive processes. Improvements to the measurement procedure were found to reduce fluorescence signal variability, enabling improved measurement precision compared to previous studies. Results show the application of treatments which serve to increase lactic acid production by the COC, and thus induce an acidification of the local microenvironment, are detectable by the pH sensor. This optical technology presents a promising platform for the measurement of pH and the detection of other extracellular biomarkers to assess cell health during assisted reproduction.

Paper Details

Date Published: 30 December 2019
PDF: 2 pages
Proc. SPIE 11202, Biophotonics Australasia 2019, 112020F (30 December 2019); doi: 10.1117/12.2541300
Show Author Affiliations
Georgina M. Sylvia, ARC Ctr. of Excellence for Nanoscale Biophotonics (Australia)
Institute for Photonics and Advanced Sensing (Australia)
Erik P. Schartner, ARC Ctr. of Excellence for Nanoscale Biophotonics (Australia)
Institute for Photonics and Advanced Sensing (Australia)
Hanna J. McLennan, ARC Ctr. of Excellence for Nanoscale Biophotonics (Australia)
Univ. of Adelaide (Australia)
Avishkar Saini, ARC Ctr. of Excellence for Nanoscale Biophotonics (Australia)
Univ. of Adelaide (Australia)
Kylie R. Dunning, ARC Ctr. of Excellence for Nanoscale Biophotonics (Australia)
Univ. of Adelaide (Australia)
Malcolm S. Purdey, ARC Ctr. of Excellence for Nanoscale Biophotonics (Australia)
Andrew D. Abell, ARC Ctr. of Excellence for Nanoscale Biophotonics (Australia)
Institute for Photonics and Advanced Sensing (Australia)
Jeremy G. Thompson, ARC Ctr. of Excellence for Nanoscale Biophotonics (Australia)
Univ. of Adelaide (Australia)


Published in SPIE Proceedings Vol. 11202:
Biophotonics Australasia 2019
Ewa M. Goldys; Brant C. Gibson, Editor(s)

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