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Proceedings Paper

Quantum diffraction unlimited protocol for single-photon fluorophores
Author(s): Josef G. Worboys; Daniel W. Drumm; Andrew D. Greentree
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Paper Abstract

Achieving higher resolution scales in optical microscopy allow a more rigorous investigation into the detailed components of cell systems. This higher resolution is typically achieved through super-resolution techniques utilizing methods inside the wave-like nature of light such as point spread function shaping and fluorophore switching. We wish to leverage both particle-like and wave-like natures of light to make a diffraction unlimited protocol. Our protocol uses the well known Hanbury Brown and Twiss (HBT) apparatus in combination with a customized second-order cross-correlation protocol. By performing least squares fitting of the HBT and intensity measurements we obtain diffraction unlimited localization for two particles of unknown relative brightness from few measurement locations. Our results show super-resolution enhancement by an order of magnitude after 5000 detection lifetimes.

Paper Details

Date Published: 30 December 2019
PDF: 2 pages
Proc. SPIE 11202, Biophotonics Australasia 2019, 112021N (30 December 2019); doi: 10.1117/12.2541217
Show Author Affiliations
Josef G. Worboys, RMIT Univ. (Australia)
Daniel W. Drumm, RMIT Univ (Australia)
Andrew D. Greentree, RMIT Univ. (Australia)

Published in SPIE Proceedings Vol. 11202:
Biophotonics Australasia 2019
Ewa M. Goldys; Brant C. Gibson, Editor(s)

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