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Proceedings Paper

Molecular imaging of extracellular vesicles in vitro via Raman metabolic labelling (Conference Presentation)
Author(s): Conor C. Horgan; Anika Nagelkerke; Thomas E. Whittaker; Valeria Nele; Lucia Massi; Ulrike Kauscher; Jelle Penders; Mads S. Bergholt; Steve R. Hood; Molly M. Stevens

Paper Abstract

Extracellular vesicles (EVs) are biologically derived nanovectors important for intercellular communication and trafficking, yet understanding of their underlying biological mechanisms remains poor. Advances have been hampered by both the complex biological origins of EVs and a lack of suitable imaging techniques. Here, we present a strategy for simultaneous in vitro imaging and molecular characterisation of EVs in 2D and 3D based on Raman spectroscopy and minimally-obstructive metabolic deuterium labelling. Metabolically-incorporated deuterium acts as a bio-orthogonal Raman-active tag for direct Raman identification of EVs and provides insights into their biocomposition and trafficking, with implications for their development as therapeutic delivery vectors.

Paper Details

Date Published: 10 March 2020
Proc. SPIE 11251, Label-free Biomedical Imaging and Sensing (LBIS) 2020, 112511G (10 March 2020); doi: 10.1117/12.2541025
Show Author Affiliations
Conor C. Horgan, Imperial College London (United Kingdom)
Anika Nagelkerke, Imperial College London (United Kingdom)
Thomas E. Whittaker, Imperial College London (United Kingdom)
Valeria Nele, Imperial College London (United Kingdom)
Lucia Massi, Imperial College London (United Kingdom)
Ulrike Kauscher, Imperial College London (United Kingdom)
Jelle Penders, Imperial College London (United Kingdom)
Mads S. Bergholt, Imperial College London (United Kingdom)
Steve R. Hood, GlaxoSmithKline (United Kingdom)
Molly M. Stevens, Imperial College London (United Kingdom)

Published in SPIE Proceedings Vol. 11251:
Label-free Biomedical Imaging and Sensing (LBIS) 2020
Natan T. Shaked; Oliver Hayden, Editor(s)

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