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Visualization of biological nano-objects with the help of multiphoton microscopy
Author(s): Olga A. Zakharova; Helena A. Zhambalova; Natalia In. Yunusova; Viktor V. Nikolaev; Alexey V. Borisov
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Paper Abstract

The results of studies of exosome visualization obtained using multiphoton fluorescence spectroscopy for healthy volunteers and patients with colorectal cancer are presented. Standard centrifuging technology was used to isolate exosomes from blood plasma. Exosome samples were investigated using the femtosecond laser pumping at the wavelength of 760 nm with and without FITC (Fluorescein isothiocyanate) labels. In particular, the exosomes with CD9 surface glycoprotein of the tetraspanin family was shown to be studied without labeling using FLIM (fluorescent lifetime microscopy).

Paper Details

Date Published: 18 December 2019
PDF: 5 pages
Proc. SPIE 11208, 25th International Symposium on Atmospheric and Ocean Optics: Atmospheric Physics, 112080G (18 December 2019); doi: 10.1117/12.2540609
Show Author Affiliations
Olga A. Zakharova, National Research Tomsk State Univ. (Russian Federation)
Institute of Strength Physics and Materials Science (Russian Federation)
Helena A. Zhambalova, Tomsk National Research Medical Ctr. (Russian Federation)
Natalia In. Yunusova, Tomsk National Research Medical Ctr. (Russian Federation)
Viktor V. Nikolaev, National Research Tomsk State Univ. (Russian Federation)
Institute of Strength Physics and Materials Science (Russian Federation)
Alexey V. Borisov, National Research Tomsk State Univ. (Russian Federation)
Siberian State Medical Univ. (Russian Federation)


Published in SPIE Proceedings Vol. 11208:
25th International Symposium on Atmospheric and Ocean Optics: Atmospheric Physics
Oleg A. Romanovskii; Gennadii G. Matvienko, Editor(s)

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