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Estimation of the collagen and elastin condition at lymphedema using multiphoton microscopy
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Paper Abstract

Methods of two-photon microscopy are widely used in the study of biological objects, in particular, skin, due to the possibility to study objects both on the surface and at depth without attracting additional fluorophores due to endogenous autofluorescence. In this paper, the methods of image analysis of the AF signal and SHG signal are applied to assess the condition of the skin during the development of lymphedema. It is shown that for groups of healthy tissue and lymphedematous using SAAID distribution histograms, changes in tissues can be detected.

Paper Details

Date Published: 18 December 2019
PDF: 5 pages
Proc. SPIE 11208, 25th International Symposium on Atmospheric and Ocean Optics: Atmospheric Physics, 112080E (18 December 2019); doi: 10.1117/12.2540604
Show Author Affiliations
Viktor V. Nikolaev, Institute of Strength Physics and Materials Science (Russian Federation)
National Research Tomsk State Univ. (Russian Federation)
Ekaterina A. Sandykova, National Research Tomsk State Univ. (Russian Federation)
Siberian State Medical Univ. (Russian Federation)
Oksana S. Kurochkina, Institute of Microsurgery (Russian Federation)
Denis A. Vrazhnov, Institute of Strength Physics and Materials Science (Russian Federation)
National Research Tomsk State Univ. (Russian Federation)
Natalia A. Krivova, National Research Tomsk State Univ. (Russian Federation)
Yury V. Kistenev, National Research Tomsk State Univ. (Russian Federation)
Institute of Microsurgery (Russian Federation)
Elena S. Sim, National Research Tomsk State Univ. (Russian Federation)
Siberian State Medical Univ. (Russian Federation)


Published in SPIE Proceedings Vol. 11208:
25th International Symposium on Atmospheric and Ocean Optics: Atmospheric Physics
Oleg A. Romanovskii; Gennadii G. Matvienko, Editor(s)

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