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Proceedings Paper

Array-based differential sensing of cancer cells using DNA-templated silver nanoclusters
Author(s): Soonwoo Hong; Yu-An Kuo; Trung Nguyen; Yuan-I Chen; Yen-Liang Liu; Prathyush Shankar; Hsin-Chih Yeh
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Paper Abstract

While differential sensing has the potential to discriminate millions to trillions of analytes based on hundreds of sensing elements, most of the current differential sensor arrays have limited scalability, with only a handful of recognition elements demonstrated. Here we demonstrate a novel differential sensor array based on a class of organic/inorganic hybrid fluorophores, DNA-templated silver nanoclusters (DNA/Ag NCs), that not only has great scalability but also combine both recognition element and signal transduction element into one unit. Six DNA/Ag NC sensors were designed to classify the lysates of six breast epithelial and cancer cell lines with distinct metastatic potential. While MDA-MB-231 and MCF7 lysates showed significantly different fluorescence signatures upon interaction with the sensor array, other cell lines were also differentiable. Our method is simple, versatile, low-cost, reliable, and scalable.

Paper Details

Date Published: 21 February 2020
PDF: 8 pages
Proc. SPIE 11254, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII, 112540Z (21 February 2020); doi: 10.1117/12.2540132
Show Author Affiliations
Soonwoo Hong, The Univ. of Texas at Austin (United States)
Yu-An Kuo, The Univ. of Texas at Austin (United States)
Trung Nguyen, The Univ. of Texas at Austin (United States)
Yuan-I Chen, The Univ. of Texas at Austin (United States)
Yen-Liang Liu, The Univ. of Texas at Austin (United States)
Prathyush Shankar, The Univ. of Texas at Austin (United States)
Hsin-Chih Yeh, The Univ. of Texas at Austin (United States)


Published in SPIE Proceedings Vol. 11254:
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVII
Dror Fixler; Ewa M. Goldys, Editor(s)

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