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Proceedings Paper

Upright aNd inverted polygon microscope (UNI-SCOPE)
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Paper Abstract

Cellular imaging in living animal has opened up a wide range of avenues to study cells in its microenvironment. High speed laser scanning microscopy possess the ability to observe fast real-time biological phenomena such as cell movements, cell division, cells death. Due to the anatomical difference of difference organs in small animals, there is a need to engineer a flexible microscope that can readily adapt to different imaging position. For videorate imaging, the design of a flexible microscope depends mainly on scanning devices. Existing multiphoton microscope platforms (i.e. Thorlabs Bergamo® II Series) uses a rotating objective mount to conform of the specimens. This is possible because of the compact resonant mirror scanners. However, for varying imaging speed using a polygon microscope, this approach is not feasible due to high rotating speed. As such, we developed a dual objective microscope system that can achieve both upright and inverted, we termed it as UNI-SCOPE. The integrated platform can achieve flexible scanning speed of up to 120 FPS with an overall footprint of 450mm*600mm*450mm. Using a dual objective approach, users can tailor the platform to the imaging sample.

Paper Details

Date Published: 30 December 2019
PDF: 2 pages
Proc. SPIE 11202, Biophotonics Australasia 2019, 112021I (30 December 2019); doi: 10.1117/12.2539970
Show Author Affiliations
Tao Xu, The Australian National Univ. (Australia)
Yongxiao Li, The Australian National Univ. (Australia)
Woei Ming Lee, The Australian National Univ. (Australia)

Published in SPIE Proceedings Vol. 11202:
Biophotonics Australasia 2019
Ewa M. Goldys; Brant C. Gibson, Editor(s)

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