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Proceedings Paper

Comparative study of oral dysplasia by conventional and surface enhanced Raman spectroscopy of whole saliva
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Paper Abstract

Oral cancer has a poor prognosis of only 50% even in the light of current technological advances. This poor prognosis may be attributed to the still unmet clinical need to diagnose oral pre-cancer and dysplasia. Raman spectroscopy, which can detect subtle biochemical changes, has been explored for the diagnosis of cancer. This study aims to address the clinical need by exploiting the high amplification factor of Surface Enhanced Raman Spectroscopy (SERS) to analyse the saliva samples of 10 healthy controls and 10 patients with oral dysplasia. Furthermore, this technique was compared to conventional Raman spectroscopy. The saliva samples were centrifuged at 14000g for 15 minutes and the supernatant was applied directly on the SERS substrate and dried. Simultaneously, the saliva samples were prepared in the same way on slides for conventional Raman analysis. A peak at 2108 cm-1, attributed to salivary thiocyanate was present in all samples from dysplasia subjects but absent in samples from healthy non-smoking subjects. Partial least squares – discriminant analysis models for classification of oral pre-cancer were developed for both Raman spectroscopy and SERS to discriminate between healthy, mild and moderate dysplasia cohorts.

Paper Details

Date Published: 21 February 2020
PDF: 9 pages
Proc. SPIE 11236, Biomedical Vibrational Spectroscopy 2020: Advances in Research and Industry, 112360A (21 February 2020); doi: 10.1117/12.2539545
Show Author Affiliations
Amuthachelvi Daniel, Technological Univ. Dublin (Ireland)
Genecy Calado, Technological Univ. Dublin (Ireland)
Isha Behl, Technological Univ. Dublin (Ireland)
Stephen Flint, Dublin Dental Univ. Hospital (Ireland)
Sheila Galvin, Dublin Dental Univ. Hospital (Ireland)
Claire Healy, Dublin Dental Univ. Hospital (Ireland)
Hugh J. Byrne, Technological Univ. Dublin (Ireland)
Fiona M. Lyng, Technological Univ. Dublin (Ireland)

Published in SPIE Proceedings Vol. 11236:
Biomedical Vibrational Spectroscopy 2020: Advances in Research and Industry
Wolfgang Petrich; Zhiwei Huang, Editor(s)

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