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Proceedings Paper

Software package for off-axis digital holographic microscopy imaging processing
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Paper Abstract

Digital Holographic Microscopy (DHM) has emerged as a powerful imaging tool due to its ability to provide quantitative sample morphological data in a label-free manner. Off-axis holography further opens possibilities of numerical approaches to image analysis and data processing. However, this involves computationally heavy workflows that limit the tool’s usability in biomedical research. In this paper, a software package is developed for off-axis hologram reconstruction and processing. The software can perform a variety of bioimaging operations including imaging, region of interest selection, automated reconstruction, and data extraction, with a user-friendly graphical user interface. Originally programmed in MATLAB, the software can be installed to use in common lab-based Microsoft Windows computers. The software can be easily adopted to work with customized off-axis DHM setups, aiming to increase the efficiency of DHM’s bioimaging workflow in the community. To demonstrate this software, blood platelet experiments were conducted to show quantitative volume change of thrombus formation.

Paper Details

Date Published: 30 December 2019
PDF: 3 pages
Proc. SPIE 11202, Biophotonics Australasia 2019, 112021C (30 December 2019); doi: 10.1117/12.2539541
Show Author Affiliations
Tienan Xu, The Australian National Univ. (Australia)
Xuefei He, The Australian National Univ. (Australia)
Zhiduo Zhang, The Australian National Univ. (Australia)
Samantha Montague, The Australian National Univ. (Australia)
Elizabeth Gardiner, The Australian National Univ. (Australia)
Woie Ming Lee, The Australian National Univ. (Australia)

Published in SPIE Proceedings Vol. 11202:
Biophotonics Australasia 2019
Ewa M. Goldys; Brant C. Gibson, Editor(s)

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