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Proceedings Paper

In vivo nano-scale vibrometry in apical-basal ends of contractile outer hair cells in the mammalian cochlea by supercontinuum source spectral-domain OCT
Author(s): Fumiaki Nin; Samuel Choi; Takeru Ota; Hiroshi Hibino
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Paper Abstract

The cochlea of the inner ear transduces sound energy into electrical signals that are essential for audition. This transduction is processed in nano-scale vibration of the cochlear sensory epithelia. In mammals, the epithelia contain various cells and structures: inner hair cells, outer hair cells (OHCs), Deiters’ cells, basilar membrane, reticular lamina, etc. The sound elicits vibration in all these constituents. Among them, only OHCs cell body actively and periodically changes in length in association with the vibration. The unique mechanical activity of OHCs modifies the sound elicited vibration in the epithelia with a feedback mechanism. Although the modification is considered to critically contribute to the high sensitivity and sharp tuning in hearing through sensory IHCs, the real motion of OHCs remains uncertain. Vibrometrical studies of cochlear mechanics has revealed important vibration of the cell bodies involving the epithelia. However, difference in vibration pattern of the apical and basal ends of the cell has remain uncertain due to low spatial resolution of the system and low reflectivity of the cells. We performed a spectral domain OCT (SD-OCT) vibrometry by using the modified commercial SD-OCT system. Because the broad spectral bandwidth and strong power of the light source improve a performance of OCT systems in both of imaging and vibrometry, we introduced a supercontinuum light source into the commercial system. Our system achieved cellular-level tomographic imaging and subnano-scale vibration measurement in the transparent epithelia with the recording time of 100 ms in in vivo animal.

Paper Details

Date Published: 21 February 2020
PDF: 6 pages
Proc. SPIE 11239, Dynamics and Fluctuations in Biomedical Photonics XVII, 112390B (21 February 2020); doi: 10.1117/12.2539303
Show Author Affiliations
Fumiaki Nin, Niigata Univ. (Japan)
Samuel Choi, Niigata Univ. (Japan)
Takeru Ota, Niigata Univ. (Japan)
Hiroshi Hibino, Niigata Univ. (Japan)

Published in SPIE Proceedings Vol. 11239:
Dynamics and Fluctuations in Biomedical Photonics XVII
Valery V. Tuchin; Martin J. Leahy; Ruikang K. Wang, Editor(s)

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