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Proceedings Paper

Polyprotein: a new standard sample to home-built optical tweezers
Author(s): Shuai Li; Chunguang Hu; Yanhua Ma; Xiaotang Hu
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Paper Abstract

Optical tweezers is one of the commonly used technologies to research on protein force spectroscopy. However, whether optical tweezers system has the capability of force spectroscopy measurement at the molecular scale is vital to single molecule experiments. In this paper, we test the capability of our home-built dual-trap optical tweezers system by stretching polyprotein (NuG2)8 which is made of eight identical tandem repeats of NuG2. With the constant velocity stretching and relaxation mode, we achieve a lot of experimental data and get the contour length increment of (NuG2)8 rapidly from the unfolding processes after fitting these data. The result is consistent with existing reports, which demonstrates optical tweezers system has the force spectroscopy test ability and (NuG2)8 can be used as a new standard sample to evaluate the test performance of optical tweezers. Using polyprotein (NuG2)8 as standard sample has two advantages: stretching polyprotein can help improve the efficiency of data statistics and a large number of experiments can reduce the randomness of the system when testing.

Paper Details

Date Published: 12 March 2020
PDF: 6 pages
Proc. SPIE 11434, 2019 International Conference on Optical Instruments and Technology: Optical Systems and Modern Optoelectronic Instruments, 1143402 (12 March 2020); doi: 10.1117/12.2539281
Show Author Affiliations
Shuai Li, Tianjin Univ. (China)
Chunguang Hu, Tianjin Univ. (China)
Yanhua Ma, Tianjin Univ. (China)
Xiaotang Hu, Tianjin Univ. (China)


Published in SPIE Proceedings Vol. 11434:
2019 International Conference on Optical Instruments and Technology: Optical Systems and Modern Optoelectronic Instruments
Juan Liu; Baohua Jia; Xincheng Yao; Yongtian Wang; Takanori Nomura, Editor(s)

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