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Review of acousto-optical devices in advanced microscopy: From 3D scanning via super-resolution to encoded multi-beams
Author(s): P. Saggau; N. Ball; P. Bianchini; J. Brockill; W. E. Brownell; J. R. Cotton; A. Diaspro; M. Duocastella; O. Gliko; V. Iyer; N. Orlova; G. D. Reddy; A. S. Tolias; D. Tsyboulski
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Paper Abstract

This review presents a selection of advanced microscope designs employing acousto-optical deflectors (AODs). In the designs covered, AODs are used as tunable diffraction gratings to control complex illumination patterns at the Fourier plane of an objective lens. This approach allows us to generate desired illumination patterns at the focal plane of a light microscope. In simple terms, I will describe two established designs, the 3D Random-Access Multi-Photon Microscope and the Standing-Wave Super-Resolution Microscope, as well as two new schemes including the Random-Access STED Microscope and the Frequency-Encoded Multi-Beam Microscope. All instruments mentioned here were designed to overcome the throughput limitations of previously used light microscopes in experimental Neuroscience.

Paper Details

Date Published: 11 November 2019
PDF: 10 pages
Proc. SPIE 11210, Fourteenth School on Acousto-Optics and Applications, 112100P (11 November 2019); doi: 10.1117/12.2538419
Show Author Affiliations
P. Saggau, Baylor College of Medicine (United States)
Allen Institute for Brain Science (United States)
Italian Institute of Technology (Italy)
N. Ball, Allen Institute for Brain Science (United States)
P. Bianchini, Italian Institute of Technology (Italy)
J. Brockill, Allen Institute for Brain Science (United States)
W. E. Brownell, Baylor College of Medicine (United States)
J. R. Cotton, Baylor College of Medicine (United States)
A. Diaspro, Italian Institute of Technology (Italy)
M. Duocastella, Italian Institute of Technology (Italy)
O. Gliko, Baylor College of Medicine (United States)
Allen Institute for Brain Science (United States)
V. Iyer, Baylor College of Medicine (United States)
N. Orlova, Allen Institute for Brain Science (United States)
G. D. Reddy, Baylor College of Medicine (United States)
A. S. Tolias, Baylor College of Medicine (United States)
D. Tsyboulski, Allen Institute for Brain Science (United States)


Published in SPIE Proceedings Vol. 11210:
Fourteenth School on Acousto-Optics and Applications
Ireneusz Grulkowski; Bogumił B. J. Linde; Martí Duocastella, Editor(s)

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