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Proceedings Paper

Frequency shifting confocal microscopy via azimuthally polarized Bessel-Gaussian beam
Author(s): Yong Liu; Cuifang Kuang
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Paper Abstract

A new frequency-shifting confocal microscopy (FSCM) illuminated by an azimuthally polarized Bessel-Gaussian (BG) beam is investigated. The solid excitation spot is produced by the BG beam modulated by a spiral phase plate, and the donut excitation spot is directly obtained by the same BG beam. Through vector diffraction theory and two-view RL reconstruction algorithm, the optical transfer functions of two confocal imaging modes and the simulation imaging of FSCM are presented. The results show that, two illumination modes produced by the azimuthally polarized BG beam can enhance the spatial resolution of FSCM, the spatial resolution of reconstructed image is mainly depended on the illumination mode with higher frequency transfer efficiency, the small pinhole is helpful to improve the contrast and spatial resolution of image. When the iterations number is about 100, the reconstructed image has good quality. This FSCM is helpful to quickly realize super resolution and high contrast in cell imaging.

Paper Details

Date Published: 19 November 2019
PDF: 7 pages
Proc. SPIE 11186, Advanced Optical Imaging Technologies II, 111861B (19 November 2019); doi: 10.1117/12.2537729
Show Author Affiliations
Yong Liu, Shanghai Univ. of Electric Power (China)
Cuifang Kuang, Zhejiang Univ. (China)
Shanxi Univ. (China)

Published in SPIE Proceedings Vol. 11186:
Advanced Optical Imaging Technologies II
Xiao-Cong Yuan; P. Scott Carney; Kebin Shi; Michael G. Somekh, Editor(s)

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