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Proceedings Paper

Detection of singlet oxygen luminescence in skin phantom based on optical fiber detection
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Paper Abstract

Singlet oxygen (1O2) is widely considered as the major cytotoxic species generated during Type-II photochemical reaction of photodynamic therapy (PDT), and its production is crucial for the treatment outcome of PDT. However, it is still a challenging task for direct detection of 1O2 by using an optical system as its extremely weak luminescence at 1270 nm. In previous study, a high-sensitivity optical fiber detection system has been developed to measure the time-resolved 1O2 luminescence spectra. In this study, an optical phantom of skin tissue has been built to simulate the skin optical properties for research in photodynamic therapy (PDT). The phantom consists of an absorber (ink) and a scatterer (Intralipid) and phosphate buffer saline (PBS). Rose Bengal (RB) was utilized as the model photosensitizer to generate 1O2. The time-resolved 1O2 luminescence spectroscopy were measured by using a 1O2 luminescence detection system with a fiber prober in tissue phantom. Furthermore, the effect of absorption coefficient (μa) and scattering coefficient (μs) on the photosensitizer triple state lifetime (τT) and 1O2 luminescence lifetime (τD) was investigated as well. The results indicated that the integrated intensity of 1O2 luminescence decrease with the increase of μa and μs. μa has no significant effect on τT and τD. τT increase with the increase of μs, and it finally stabilized around 1.6 μs. Meanwhile, τD decrease firstly and then increase slowly, and it finally stabilized around 14 μs. This result indicates that this system for 1O2 luminescence have a potential for clinical applications in PDT dosimetry.

Paper Details

Date Published: 20 November 2019
PDF: 6 pages
Proc. SPIE 11190, Optics in Health Care and Biomedical Optics IX, 111902X (20 November 2019); doi: 10.1117/12.2537692
Show Author Affiliations
Ying Hu, Fujian Normal Univ. (China)
Lisheng Lin, Fujian Normal Univ. (China)
Lina Liu, Fujian Normal Univ. (China)
Yunfei Yu, Fujian Normal Univ. (China)
Xianglian Liao, Fujian Normal Univ. (China)
Ying Gu, Chinese PLA General Hospital (China)
Buhong Li, Fujian Normal Univ. (China)

Published in SPIE Proceedings Vol. 11190:
Optics in Health Care and Biomedical Optics IX
Qingming Luo; Xingde Li; Ying Gu; Yuguo Tang; Dan Zhu, Editor(s)

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