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Ratiometric autofluorescence lifetime imaging system standardization and application for head and neck cancer
Author(s): Peter A. Pellionisz; Yong Hu; Andy Moon; Khuzaima Rangwalla; Jake Pensa; Maie A. St. John
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Paper Abstract

Dynamic optical contrast imaging (DOCI) is a novel optical imaging technology that rapidly generates image contrast from measurements of aggregate endogenous fluorescence lifetime in a clinically meaningful field of view. Recently, our use of this system in both human ex-vivo and in-vivo specimens generated statistically significant contrast between tumor and adjacent normal tissue in biopsies taken from patients undergoing surgery for head and neck squamous cell carcinoma and primary hyperthyroidism. In this work we evaluated the components and resolution of our next-generation DOCI system. We also standardized the quantitative output of our system against the fluorescence lifetime values of three dye standards with monoexponential decay using a commercial Leica two-photon fluorescence lifetime imaging microscope. Significantly, our system continued to demonstrate clinically meaningful contrast between tissue samples with multiexponential decay in near real-time.

Paper Details

Date Published: 20 November 2019
PDF: 8 pages
Proc. SPIE 11190, Optics in Health Care and Biomedical Optics IX, 1119006 (20 November 2019); doi: 10.1117/12.2537631
Show Author Affiliations
Peter A. Pellionisz, Univ. of California, Los Angeles (United States)
Yong Hu, Univ. of California, Los Angeles (United States)
Andy Moon, Univ. of California, Los Angeles (United States)
Khuzaima Rangwalla, Univ. of California, Los Angeles (United States)
Jake Pensa, Univ. of California, Los Angeles (United States)
Maie A. St. John, Univ. of California, Los Angeles (United States)


Published in SPIE Proceedings Vol. 11190:
Optics in Health Care and Biomedical Optics IX
Qingming Luo; Xingde Li; Ying Gu; Yuguo Tang; Dan Zhu, Editor(s)

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