Share Email Print

Proceedings Paper

A tunable 3D optical diffraction tomography system with high measurement accuracy
Author(s): Xing Lv; Shuo Cao; Mi Li; Guanghui Wang; Jiabi Chen
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

We present a tunable experimental setup to obtain the three-dimensional refractive index distribution of microscopic biological structures. We introduce an adjustable system to change the position of the focal plane and perform stitched reconstruction. There are two main approaches for obtaining the projections of sample in optical diffraction tomography: beam scanning and rotating sample. Compared to beam scanning, the method of rotating sample allows the sample to be rotated 180° to capture uniformly distributed data, which improves the accuracy of the phase measurement and the resolution of the reconstruction result. The depth-of-field in the optical diffraction tomography setups is very small and the method of rotating sample inevitably causes the sample to deviate from the depth-of-field during the rotation, making it difficult to obtain ideal data. We divided the sample position deviation area into several ideal data acquisition areas and collected the ideal data in each area by shifting the position of the focal plane. By the combination of 180° projection method and stitched reconstruction method, we have obtained high measurement accuracy results with uniform resolution.

Paper Details

Date Published: 19 November 2019
PDF: 7 pages
Proc. SPIE 11186, Advanced Optical Imaging Technologies II, 111860E (19 November 2019); doi: 10.1117/12.2537601
Show Author Affiliations
Xing Lv, Nanjing Univ. (China)
Shuo Cao, Nanjing Univ. (China)
Mi Li, Nanjing Univ. (China)
Guanghui Wang, Nanjing Univ. (China)
Jiabi Chen, Nanjing Univ. (China)

Published in SPIE Proceedings Vol. 11186:
Advanced Optical Imaging Technologies II
Xiao-Cong Yuan; P. Scott Carney; Kebin Shi; Michael G. Somekh, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?