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Proceedings Paper

Multiphoton imaging of perineural invasion in breast cancer
Author(s): Wenjiao Ren; Wenhui Guo; Deyong Kang; Chuan Wang; Jianxin Chen; Lianhuang Li
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Paper Abstract

Perineural invasion (PNI) is the process of neoplastic invasion of nerves. The nerve sheath includes three layers, that is, the epineurium, perineurium and endoneurium from the outside to the inside. Any one of them is invaded by malignant tumor cells, or more than 33% of the circumference of the nerve should be surrounded by tumor cells, then it can be defined as perineural invasion (PNI). Perineural invasion is another way of tumor cell metastasis, which not only indicates the poor prognosis of patients but also indicates the decline of survival rate. In this study, multiphoton microscopy (MPM) which is based on the intrinsic nonlinear optical signals—two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) was applied to identify the perineural invasion in breast cancer patients, including the recognition of normal nerves and tumor cells infiltrating into the nerve. Our aim is to determine the potential feasibility of using this microscopy to identify tumor invasion around the nerves. The results mean that it is feasible to detect perineural invasion with multiphoton microscopy, and to provide a new auxiliary tool for pathologists to quickly and effectively diagnose perineural invasion.

Paper Details

Date Published: 20 November 2019
PDF: 6 pages
Proc. SPIE 11190, Optics in Health Care and Biomedical Optics IX, 111902Q (20 November 2019); doi: 10.1117/12.2537586
Show Author Affiliations
Wenjiao Ren, Fujian Normal Univ. (China)
Wenhui Guo, Fujian Medical Univ. (China)
Deyong Kang, Fujian Medical Univ. (China)
Chuan Wang, Fujian Medical Univ. (China)
Jianxin Chen, Fujian Normal Univ. (China)
Lianhuang Li, Fujian Normal Univ. (China)

Published in SPIE Proceedings Vol. 11190:
Optics in Health Care and Biomedical Optics IX
Qingming Luo; Xingde Li; Ying Gu; Yuguo Tang; Dan Zhu, Editor(s)

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