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Proceedings Paper

Imaging characteristic optimization of digital holographic microscopy for onion epidermal cells
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Paper Abstract

The optimization of image resolution for digital holographic scanning imaging of biological cells is investigated. Digital holographic scanning imaging experiments on the upper epidermal cells of onions are performed to demonstrate the validity of resolution optimization algorithm. In the experiments, the holograms of the upper epidermal cells of onion are recorded at a certain scanning rate, and then are processed by using the resolution optimization algorithm. As a result, the phase images of the onion epidermal cells with higher contrast and resolution are obtained. According to the synthetic holograms, the changes of cell nucleus and actin microfilament inside onion’s epidermal cells are displayed. In addition, the dehydration process and plasmolysis phenomenon inside onion epidermal cells are also exhibited by recording longterm scanning holograms of living epidermal cells. The experimental results demonstrate that image quality of living onion epidermal cells can be improved by optimizing the algorithms.

Paper Details

Date Published: 18 November 2019
PDF: 8 pages
Proc. SPIE 11188, Holography, Diffractive Optics, and Applications IX, 111881W (18 November 2019); doi: 10.1117/12.2537550
Show Author Affiliations
Rikang Qin, Beijing Univ. of Technology (China)
Yanyan Jia, Beijing Univ. of Technology (China)
Yiwei Liu, Beijing Univ. of Technology (China)
Hongpeng Qin, Beijing Univ. of Technology (China)
Yibo Wang, Beijing Univ. of Technology (China)
Zhuqing Jiang, Beijing Univ. of Technology (China)


Published in SPIE Proceedings Vol. 11188:
Holography, Diffractive Optics, and Applications IX
Yunlong Sheng; Changhe Zhou; Liangcai Cao, Editor(s)

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