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Graphene oxide based FRET probe for mast cell degranulation
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Paper Abstract

Degranulation in mast cell is usually characterized by the release of tryptase. We developed a fluorescence resonance energy transfer (FRET) probe based on graphene oxide (GO) to detect tryptase released from mast cells. The GO based FRET probe is composed of GO and a self-assembled complex of tryptase-specific recognition peptide chains labeled with isothiocyanate fluorescein. The fluorescence intensity around the mast cells increased when the mast cells were stimulated with C48/80, a kind of reagent promoting degranulation. The fluorescence distribution is inhomogenous. The fluorescence intensity was dependent on the concentration of C48/80 and the stimulation time. These results demonstrate that GO-base FRET probe could be used to study degranulation in mast cells.

Paper Details

Date Published: 20 November 2019
PDF: 6 pages
Proc. SPIE 11190, Optics in Health Care and Biomedical Optics IX, 111902J (20 November 2019); doi: 10.1117/12.2537508
Show Author Affiliations
Shuzhen Tang, Fujian Normal Univ. (China)
Jianshu Xu, Fujian Normal Univ. (China)
Yating Lin, Fujian Normal Univ. (China)
Mengmeng Zheng, Fujian Normal Univ. (China)
Yamin Lin, Fujian Normal Univ. (China)
Yimei Huang, Fujian Normal Univ. (China)
Shusen Xie, Fujian Normal Univ. (China)
Juqiang Lin, Fujian Normal Univ. (China)


Published in SPIE Proceedings Vol. 11190:
Optics in Health Care and Biomedical Optics IX
Qingming Luo; Xingde Li; Ying Gu; Yuguo Tang; Dan Zhu, Editor(s)

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