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Proceedings Paper

Non-invasive 3D real time observation of physiological traits during the embryonic development of insects
Author(s): Hao Tan; Ya Su; Liya Wei; X. Steve Yao; Tongtong Mai; Xu Li
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Paper Abstract

The visualization of embryonic development is important for the study of basic physiological development. It is also indispensable for evaluating the effects of modification and editing of genetic engineering. Compared with traditional invasive methods of monitoring embryo development, optical coherence tomography (OCT) has its great advantages, such as high resolution, real time and non-invasive. These characteristics of OCT make it possible to observe a living embryo in its whole growth development. In this paper, we use OCT to monitor the embryonic development of locusts noninvasively and reconstruct the two-dimensional and three-dimensional image of the embryo at different development stages. The phenomena of anatrepsis, revolution and katatrepsis are observed. The in-vitro observations of the prior studies with invasive methods are confirmed by the in-vivo experimental results. We hope OCT is a useful tool for the understanding of the embryonic development of insects.

Paper Details

Date Published: 20 November 2019
PDF: 6 pages
Proc. SPIE 11190, Optics in Health Care and Biomedical Optics IX, 111902F (20 November 2019); doi: 10.1117/12.2537479
Show Author Affiliations
Hao Tan, Hebei Univ. (China)
Ya Su, Hebei Univ. (China)
Liya Wei, Hebei Univ. (China)
X. Steve Yao, Hebei Univ. (China)
Tongtong Mai, Hebei Univ. (China)
Xu Li, Hebei Univ. (China)

Published in SPIE Proceedings Vol. 11190:
Optics in Health Care and Biomedical Optics IX
Qingming Luo; Xingde Li; Ying Gu; Yuguo Tang; Dan Zhu, Editor(s)

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