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Proceedings Paper

Holographic optical element based digital holographic interferometer for label-free imaging of staphylococcus aureus bacteria
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Paper Abstract

Staphylococcus aureus (S. aureus) is a round-shaped, aggressive human pathogen that can grow either by fermentation or by utilizing an elective terminal electron acceptor without oxygen. These bacteria can spread from an infected person to others, and it can enter into the body via the bloodstream and can infect body parts and organs. To avoid spreading infections and life-threatening diseases, a rapid, non-invasive, non-contact expeditious detection system is required. In this paper, a holographic optical element based digital holographic interferometric (DHI) system has been demonstrated for the label-free imaging of S. aureus bacteria. A comparison has been made in the proposed holographic optical element based DHI system and the conventional off-axis Mach–Zehnder configuration based DHI system. The proposed DHI system is an economical, efficient and easy-to-operate interferometric system, and significantly improves the signalto- noise ratio of recorded digital holograms without any spatial filtering.

Paper Details

Date Published: 18 November 2019
PDF: 8 pages
Proc. SPIE 11188, Holography, Diffractive Optics, and Applications IX, 111881U (18 November 2019); doi: 10.1117/12.2537317
Show Author Affiliations
Vivek Rastogi, Indian Institute of Technology Delhi (India)
Shilpi Agarwal, Indian Institute of Technology Delhi (India)
Rahul Gadkari, Indian Institute of Technology Delhi (India)
Chandra Shakher, Indian Institute of Technology Delhi (India)


Published in SPIE Proceedings Vol. 11188:
Holography, Diffractive Optics, and Applications IX
Yunlong Sheng; Changhe Zhou; Liangcai Cao, Editor(s)

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