Share Email Print

Proceedings Paper

Rapid cell phase imaging using quantitative interferometric microscopy with fast shearing phase unwrapping method
Author(s): Xi Zhang; Zhilong Jiang; Xiaoliang He; Yan Kong; Cheng Liu; Shouyu Wang
Format Member Price Non-Member Price
PDF $17.00 $21.00

Paper Abstract

Cell observations and analysis are often required in biological and medical fields for cell condition evaluations and disease diagnostics. Compared to classical methods such as bright/dark field microscopy and fluorescent microscopy, quantitative interferometric microscopy as one of the quantitative phase microscopic techniques is able to provide high contrast image of label free samples thus can be potentially using in live cell imaging. While it often suffers from the low processing speed often due to the heavy computational load in phase unwrapping. In order to accelerate the imaging speed, we design a rapid cell phase imaging technique relying on the fast shearing phase unwrapping method. It can extract the sample information from a single-shot interferogram showing that it can capture live cell images. Moreover, the phase unwrapping process is significantly reduced with accelerated speed. Considering the system can provide quantitative and high-contrast live cell imaging in label free mode with high accuracy, fast speed and large field of view, it can be a potential tool for cell observations and measurements in biological and medical applications.

Paper Details

Date Published: 20 November 2019
PDF: 6 pages
Proc. SPIE 11190, Optics in Health Care and Biomedical Optics IX, 111901Y (20 November 2019); doi: 10.1117/12.2537204
Show Author Affiliations
Xi Zhang, Jiangnan Univ. (China)
Zhilong Jiang, Jiangnan Univ. (China)
Xiaoliang He, Jiangnan Univ. (China)
Yan Kong, Jiangnan Univ. (China)
Cheng Liu, Jiangnan Univ. (China)
Shouyu Wang, Jiangnan Univ. (China)

Published in SPIE Proceedings Vol. 11190:
Optics in Health Care and Biomedical Optics IX
Qingming Luo; Xingde Li; Ying Gu; Yuguo Tang; Dan Zhu, Editor(s)

© SPIE. Terms of Use
Back to Top
Sign in to read the full article
Create a free SPIE account to get access to
premium articles and original research
Forgot your username?