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High-speed in vitro intensity diffraction tomography
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Paper Abstract

We demonstrate a label-free, scan-free intensity diffraction tomography technique utilizing annular illumination (aIDT) to rapidly characterize large-volume 3D refractive index distributions in vitro. By optimally matching the illumination geometry to the microscope pupil, our technique reduces the data requirement by 60x to achieve high-speed 10 Hz volume rates. Using 8 intensity images, we recover ∼350 x 100 x 20μm3 volumes with near diffraction-limited lateral resolution of 487 nm and axial resolution of 3.4μm. Based on the 3D quantitative reconstructions of unicellular diatom microalgae, epithelial buccal cell clusters with native bacteria, and live Caenorhabditis elegans specimens, aIDT shows promise as a powerful high-speed, label-free computational microscopy technique for these applications where natural imaging is required to evaluate environmental effects on a sample in real-time.

Paper Details

Date Published: 19 November 2019
PDF: 9 pages
Proc. SPIE 11186, Advanced Optical Imaging Technologies II, 111860Y (19 November 2019); doi: 10.1117/12.2536918
Show Author Affiliations
Jiaji Li, Nanjing Univ. of Science and Technology (China)
Boston Univ. (United States)
Alex Matlock, Boston Univ. (United States)
Yunzhe Li, Boston Univ. (United States)
Qian Chen, Nanjing Univ. of Science and Technology (China)
Chao Zuo, Nanjing Univ. of Science and Technology (China)
Lei Tian, Boston Univ. (United States)


Published in SPIE Proceedings Vol. 11186:
Advanced Optical Imaging Technologies II
Xiao-Cong Yuan; P. Scott Carney; Kebin Shi; Michael G. Somekh, Editor(s)

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