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Proceedings Paper

Two-photon excited polarized fluorescence in NADH in methanol/water solutions
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Paper Abstract

We studied polarized fluorescence in biological coenzyme NADH in water-methanol solutions upon two-photon excitation with femtosecond laser pulses at 720 nm. The polarized fluorescence decay was recorded by a time correlated single photon counting (TCSPC) system. Fluorescence decay times, rotational diffusion time, fluorescence anisotropy, and the ratio of two pre-exponential factors have been determined and studied as a function of methanol concentration. The results obtained were interpreted on the basis of a model of NADH denaturation processes in solutions and can be used for modeling of NADH binding with various dehydrogenases in living cells.

Paper Details

Date Published: 22 July 2019
PDF: 6 pages
Proc. SPIE 11076, Advances in Microscopic Imaging II, 1107621 (22 July 2019); doi: 10.1117/12.2536641
Show Author Affiliations
I. A. Gorbunova, Ioffe Institute (Russian Federation)
St. Petersburg Academic Univ. (Russia)
N. O. Bezverkhnii, Ioffe Institute (Russian Federation)
Peter the Great St. Petersburg Polytechnic Univ. (Russia)
A. A. Zhikhoreva, Ioffe Institute (Russian Federation)
Y. M. Beltukov, Ioffe Institute (Russian Federation)
M. E. Sasin, Ioffe Institute (Russian Federation)
J. Rubayo-Soneira, Univ. de La Habana (Cuba)
O. S. Vasyutinskii, Ioffe Institute (Russian Federation)


Published in SPIE Proceedings Vol. 11076:
Advances in Microscopic Imaging II
Emmanuel Beaurepaire; Francesco Saverio Pavone; Peter T. C. So, Editor(s)

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