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Label-free imaging of lymph nodes with stimulated Raman scattering microscopy
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Paper Abstract

Lymph nodes are an important secondary lymphoid organ in the lymphatic system. It is the major site of B cells, T cells and other white blood cells, leading to the function of identifying and fighting infection. To observe the structure of the lymph node, it is necessary to label it with dyes or fluorescent probes. Multi-color labeling are required to label various cell types and structures, which may be difficult under conditions such as in vivo labeling. Stimulated Raman scattering microscopy (SRS) is a burgeoning label-free biological imaging technique that has been used in many areas, such as tumor detection, pharmacokinetics, and neuroscience. Here, we developed a method of label-free imaging of various cell types and structures in lymph nodes with SRS. Collagenous fibers in the lymph node capsule, subcapsular sinus macrophages, B cells, blood vessels and white blood cells were observed in fresh lymph nodes. Based on the visualization of lymph node structure, we prospected its applications in cancer research, aging research and extracellular matrix research.

Paper Details

Date Published: 20 November 2019
PDF: 6 pages
Proc. SPIE 11190, Optics in Health Care and Biomedical Optics IX, 111900J (20 November 2019); doi: 10.1117/12.2536264
Show Author Affiliations
Yuanzhen Suo, Peking Univ. (China)
Wenlong Yang, Harvard Univ. (United States)
Fake Lu, Harvard Univ. (United States)
Xiaoliang Sunney Xie, Peking Univ. (China)


Published in SPIE Proceedings Vol. 11190:
Optics in Health Care and Biomedical Optics IX
Qingming Luo; Xingde Li; Ying Gu; Yuguo Tang; Dan Zhu, Editor(s)

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