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Proceedings Paper

Feature analysis of wrapped phases of nucleated cells in polar coordinates
Author(s): Qibao Shen; Yuanyuan Xu; Weifeng Jin; Hao Han; Zhaomin Liu; Lingxuan Zhang; Yawei Wang
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Paper Abstract

Digital phase microscopy has made great progress in the study of morphology and dynamics of biological cells, especially the homogeneous cells. For the heterogeneous nucleated cells with relatively complex internal structures, there are still few methods of phase unwrapping suitable for observation and measurement. In this paper, a method is proposed to identify the substructure of cells only with their wrapped phases. According to the structural features of real nucleated cells, their similar models are established firstly, and then their wrapped phases are obtained in Cartesian coordinates and polar coordinates. Based on the relationship between the parameters of the two coordinate systems, the pattern recognition and image processing techniques are used to analyze the parcel phase of the single-core and multi-core, regular and irregular kernels, and the central and boundary kernel models. Then, the texture shape distribution, the texture overlap condition, the opening and closing state of the equal phase line, the peak number and the peak position are selected as reference features, and these reference features are combined and optimized as the judgment condition for identifying the nuclear structure model, and the wrapped phase in polar coordinates is screened and identified, which effectively distinguishes three typical types of nuclear cell models. It provides a new, unpacked method for the classification and identification the substructures of the nucleated cells.

Paper Details

Date Published: 17 May 2019
PDF: 7 pages
Proc. SPIE 11170, 14th National Conference on Laser Technology and Optoelectronics (LTO 2019), 1117039 (17 May 2019);
Show Author Affiliations
Qibao Shen, Jiangsu Univ. (China)
Yuanyuan Xu, Jiangsu Univ. (China)
Nanjing Univ. (China)
Weifeng Jin, Jiangsu Univ. (China)
Hao Han, Jiangsu Univ. (China)
Zhaomin Liu, Jiangsu Univ. (China)
Lingxuan Zhang, Jiangsu Univ. (China)
Yawei Wang, Jiangsu Univ. (China)


Published in SPIE Proceedings Vol. 11170:
14th National Conference on Laser Technology and Optoelectronics (LTO 2019)
Jianqiang Zhu; Weibiao Chen; Zhenxi Zhang; Minlin Zhong; Pu Wang; Jianrong Qiu, Editor(s)

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