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Proceedings Paper

High-resolution IR-based imaging with third-order sum-frequency generation microscopy (Conference Presentation)

Paper Abstract

We studied the use of vibrationally resonant, third-order sum-frequency generation (TSFG) for imaging of biological samples. We found that laser-scanning TSFG provides vibrationally sensitive imaging capabilities of lipid droplets and structures in sectioned tissue samples. Although the contrast is based on the infrared-activity of molecular modes, TSFG images exhibit a high lateral resolution of 0.5 μm or better. We observed that the imaging properties of TSFG resemble the imaging properties of coherent anti-Stokes Raman scattering (CARS) microscopy, offering a nonlinear infrared alternative to coherent Raman methods. TSFG microscopy holds promise as a high-resolution imaging technique in the fingerprint region where coherent Raman techniques often provide insufficient sensitivity.

Paper Details

Date Published: 9 September 2019
Proc. SPIE 11122, Ultrafast Nonlinear Imaging and Spectroscopy VII, 111220I (9 September 2019); doi: 10.1117/12.2530523
Show Author Affiliations
Eric O. Potma, Univ. of California, Irvine (United States)
Adam M. Hanninen, Univ. of California, Irvine (United States)
Richard C. Prince, Univ. of California, Irvine (United States)

Published in SPIE Proceedings Vol. 11122:
Ultrafast Nonlinear Imaging and Spectroscopy VII
Zhiwen Liu; Demetri Psaltis; Kebin Shi, Editor(s)

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