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Proceedings Paper

Nanophotonic tools based on the conjugates of nanoparticles with the single-domain antibodies for multi-photon micrometastases detection and ultrasensitive biochemical assays
Author(s): Alyona Sukhanova; Fernanda Ramos-Gomes; Patrick Chames; Daniel Baty; Frauke Alves; Pavel Samokhvalov; Igor Nabiev
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Paper Abstract

Semiconductor quantum dots (QDs) are characterized by orders of magnitude higher multiphoton linear absorption cross-sections compared with conventional organic dyes. Combined with the QD photoluminescence quantum yield approaching 100%, this fact opens great prospects for the twophoton functional tumor imaging with QDs tagged with highly specific recognition molecules. Single-domain antibodies (sdAbs) or “nanobodies” derived from lamas are the smallest high-affinity recognition molecules, which may be tagged with the QDs thus permitting not only solid tumors multiphoton imaging but also rare disseminated cancer cells and micrometastases in the depth of the tissue to be detected. Additionally, unique photostability of QDs enables signal accumulation and significant enhancement of the sensitivity of routine biochemical and immunohistochemical assays to be obtained when the conjugates of QDs, instead of organic dyes, are used.

Paper Details

Date Published: 3 October 2019
PDF: 5 pages
Proc. SPIE 11207, Fourth International Conference on Applications of Optics and Photonics, 112072A (3 October 2019);
Show Author Affiliations
Alyona Sukhanova, Lab. de Recherche en Nanosciences, LRN, Univ. de Reims Champagne-Ardenne (France)
National Research Nuclear Univ. MEPhI (Russian Federation)
Fernanda Ramos-Gomes, Max Planck Institute for Experimental Medicine (Germany)
Göttingen Univ. Medical Ctr. (Germany)
Patrick Chames, Aix Marseille Univ., CNRS, INSERM, Institut Paolo-Calmettes, CRCM (France)
Daniel Baty, Aix Marseille Univ., CNRS, INSERM, Institut Paolo-Calmettes, CRCM (France)
Frauke Alves, Max Planck Institute for Experimental Medicine (Germany)
Göttingen Univ. Medical Ctr. (Germany)
Pavel Samokhvalov, National Research Nuclear Univ. MEPhI (Russian Federation)
Igor Nabiev, Lab. de Recherche en Nanosciences, LRN, Univ. de Reims Champagne-Ardenne (France)


Published in SPIE Proceedings Vol. 11207:
Fourth International Conference on Applications of Optics and Photonics
Manuel Filipe P. C. M. Martins Costa, Editor(s)

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